Islet cell exocytosis

Department of Clinical Sciences Malmö

Autumn 2015

Head

Group members

Jones Ofori, PhD student
Anna Edlund, Postdoc Fellow
Mototsugu Nagao
, Postdoc Fellow
Anna Wendt, Associate Researcher
Jonathan LS Esguerra
, Associate Researcher
Anna-Maria Veljanovska Ramsay, Lab technician
Britt-Marie S Nilsson, Lab technician

Former members

Helena Malm, PhD student
Inês G. Mollet, Associate Researcher
Vishal A. Salunkhe, PhD student
Sofia A Andersson, PhD student
Morten Gram Pedersen
, Post-doc
Yang de Marinis
, PhD student
Jenny Vikman, PhD student

Research overview

Type-2 diabetes (T2D) is a complex disease dependent on both genetic background and life-style factors. The disease is associated with increase in blood glucose level which is due to reduced insulin secretion from the pancreatic β-cell and impaired insulin action at the target cells.  Diabetes is also associated with a perturbed glucagon secretion. The main focus of our research is to investigate the regulation of insulin and glucagon secretion from a cell physiological perspective and with a specific interest in how non-coding RNAs (ncRNA) are involved in this regulation.  Belonging to the group of ncRNAs are microRNAs (miRNA). The function of miRNAs is to suppress the expression of genes by mRNA degradation, mRNA deadenylation and/or translational repression.

To functionally investigate exocytosis in these cells we utilize the patch-clamp technique. Exocytosis is measured as an increase in membrane capacitance.  The patch-clamp technique has the advantage that it is possible to measure on single cells with a high temporal resolution. We also combine these techniques with live confocal microscopy, hormone measurements utilizing RIA as well as necessary molecular biology techniques and protein chemistry.

Ultimately, our research gives new insight into treatment and discovery strategies of T2D, and we anticipate our novel findings will be essential for future treatment of T2D.

Source: Eliasson L and Esguerra JL. Acta Physiol (Oxf). 2014 Jun;211(2):273-84.

Last updated: November 2, 2016
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