Photo of Anna Wendt

Contact

Address

Department of Clinical Scieces Malmö, Islet Cell Exocytosis,
CRC, Entr 72, building 81, Level 11, University Hospital Malmö
SE-205 02  Malmö, Sweden

Phone

+46 40 391169

Fax

+46 40 391222

E-mail

Ines.Mollet AT med.lu.se

Ines G. Mollet

Associate Researcher

Research Focus

Gene regulatory networks and control of allostasis in diabetes

My interest lies in decyphering how impaired stress-related adaptations could be responsible for the onset and progression of diabetes and the loss of allostatic control.

The current project focuses on gene regulatory networks involved in transcriptional regulation of pancreatic islet microRNAs in response to various metabolic insults and stress signals, including analysis of pancreatic islet stimulus-secretion coupling. The project also incorporates high-throughput profiling of downstream microRNA target networks and feedback regulation.

Additional projects in collaboration with other members of the Islet Cell Exocytosis Unit focus on the role of osteopontin in pancreatic islet pathophysiology in diabetic mice and the effect of statins and diet on mouse pancreatic islet gene expression, morphology, ER and mitochondrial stress, calcium signalling and insulin granule distribution.

Latest Communications

Mollet I.G., Malm H., Anna Wendt, Orho-Melander M., Eliasson L. Calcium-Calmodulin Transcriptional Activator, a new player in the regulation of microRNAs and insulin secretion. Lund University Diabetes Centre, Dept Clinical Sciences Malmö, Lund University, Malmö, Sweden. (Presented at: Islet Study Group – ISG - 2014, Lausanne).

Malm H.A., Mollet I.G., Esguerra J.L.S., Orho-Melander M., Eliasson L. Dephosphorylation and nuclear translocation of the CREB-regulated transcription co-activator 1 (CRTC1) elevates miR-212 and miR-132 expression in insulin secreting cells. Lund University Diabetes Centre, Dept Clinical Sciences Malmö, Lund University,Malmö, Sweden. (European Association for the Study of Diabetes – EASD - 2014, Vienna).

Vishal A. Salunkhe, Sofia A. Andersson, Jonathan L.S. Esguerra, Ines G. Mollet, Anna Wendt and Lena Eliasson. Regulation of miR-335 expression contributes to orchestrate insulin secretion at the level of exocytosis in insulin secreting INS-1 832/13 cells . Islet Cell Exocytosis, Lund University Diabetes Centre, Department of Clinical Sciences Malmö, Lund University, Malmö, Sweden. (Presented at: European Association for the Study of Diabetes – EASD - 2013, Barcelona)

G. Hindy, I. G. Mollet, G.Rukh, U. Ericson, M. Orho-Melander. Several type 2 diabetes associated variants in WNT pathway associated genes interact with dietary fiber on type 2 diabetes incidence. Lund University Diabetes Centre, Department of Clinical Sciences, Lund University, Malmö, Sweden. (Presented at: European Association for the Study of Diabetes – EASD - 2013, Barcelona).

Recent publications

Christensen GL, Jacobsen ML, Wendt A, Mollet IG, Friberg J, Frederiksen KS, Meyer M, Bruun C, Eliasson L, Billestrup N. , Bone Morphogenetic Protein 4 inhibits insulin secretion from rodent beta cells through regulation of Calbindin1 expression and reduced voltage dependent calcium currents. Diabetologia. 2015 Jun;58(6):1282-90. PubMed PMID: 25828920

Salunkhe VA, Esguerra JL, Ofori JK, Mollet IG, Braun M, Stoffel M, Wendt A, Eliasson L. Modulation of microRNA-375 expression alters voltage-gated Na(+) channel properties and exocytosis in insulin-secreting cells. Acta Physiol (Oxf). 2015 Jan 28. PubMed PMID:25627423

Esguerra JL, Mollet IG, Salunkhe VA, Wendt A, Eliasson L. Regulation of Pancreatic Beta Cell Stimulus-Secretion Coupling by microRNAs. Genes (Basel). 2014 Nov 6;5(4):1018-31. Review. PubMed PMID: 25383562.

Fadista J, Vikman P, Laakso EO, Mollet IG, Esguerra JL, Taneera J, Storm P, Osmark P, Ladenvall C, Prasad RB, Hansson KB, Finotello F, Uvebrant K, Ofori JK, Di Camillo B, Krus U, Cilio CM, Hansson O, Eliasson L, Rosengren AH, Renström E, Wollheim CB, Groop L. Global genomic and transcriptomic analysis of human pancreatic islets reveals novel genes influencing glucose metabolism. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):13924-9. PubMed PMID: 25201977

Edsfeldt A, Gonçalves I, Grufman H, Nitulescu M, Dunér P, Bengtsson E, Mollet IG, Persson A, Nilsson M, Orho-Melander M, Melander O, Björkbacka H, Nilsson J. Impaired fibrous repair: a possible contributor to atherosclerotic plaque vulnerability in patients with type II diabetes. Arterioscler Thromb Vasc Biol. 2014 Sep;34(9):2143-50. PubMed PMID: 25035341.

Sharoyko VV, Abels M, Sun J, Nicholas LM, Mollet IG, Stamenkovic JA, Göhring I, Malmgren S, Storm P, Fadista J, Spégel P, Metodiev MD, Larsson NG, Eliasson L, Wierup N, Mulder H. Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes. Hum Mol Genet. 2014 Nov 1;23(21):5733-49. PubMed PMID:24916378

Reinbothe TM, Safi F, Axelsson AS, Mollet IG, Rosengren AH. Optogenetic control of insulin secretion in intact pancreatic islets with β-cell-specific expression of Channelrhodopsin-2. Islets, 2014; Feb 18;6.PubMed PMID: 25483880.

Collaborations within Lund University

• Olga Göransson, Protein Phosphorylation Unit, Lund University Diabetes Centre, Lund, Sweden
• Malin Fex, Diabetes and Celiac Disease Unit, Lund University Diabetes Centre, Malmö, Sweden
• Hindrik Mulder, Molecular Metabolism Unit, Lund University Diabetes Centre, Malmö, Sweden
• Isabel Gonçalves, Experimental and Cardiovascular Research, Lund University, Malmö, Sweden
• Anna Hultgårdh-Nilsson, Vessel Wall Biology Unit, Experimental Medical Science, Lund University, Lund, Sweden

External Collaborations

• Thomas Reinbothe, University of Gothenburg, Sahlgrenska Academy, Inst. Neuroscience & Physiology, Göteborg, Sweden
• Romano Regazzi, Université de Lausanne, Switzerland
• Matthew N. Poy, Max Delbrueck Center for Molecular Medicine, Berlin, Germany
• Claire L. Shovlin, NHLI Cardiovascular Sciences, Imperial College London, UK

Academic degree

PhD (2009) in Biomedical Sciences from Faculty of Medicine, University of Lisbon, Portugal, with Prof. M. Carmo-Fonseca. Thesis – Genome Wide Mining of Alternative Splicing in Metazoan Model Organisms.

Own database

ExonMine - Database of alternative splicing (http://www.imm.fm.ul.pt/exonmine/)

Research Networks

Lund University Diabetes Centre (LUDC/Exodiab)

ORCID: http://orcid.org/0000-0002-1422-1336

ResearchGate: https://www.researchgate.net/profile/Ines_Mollet/contributions

Svenska Läkaresällskapet (Swedish Society of Medicine): www.sls.se

European Association for the Study of Diabetes (EASD)

Last updated: May 26, 2015
Website contact: LUDC webteam

LUDC, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00